You are viewing the site in preview mode

Skip to main content

Table 1 (abstract P-227). Summary of transfection conditions

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Parameters Media screen for GFP transfection Optimize of GFP transfection Comparison of transfection method
Media type ProCHO5, Opti-MEM, CHOMACS CD, ProCHO4, UltraCHO, DMEM/F12 Opti-MEM + CHOMACS CD Opti-MEM + CHOMACS CD
Transfection volume (w/o or w media addition at 5 hpt*) 5 mL 12.5 + 12.5 mL 2.5 + 2.5 mL
Transfection protocol Insitu Insitu Conventional Insitu
Cells amount 10 x 106 62 x 106 10 x 106
pEGFP-N1 plasmid pDNA (μg) 6 37.5 5 6
Linear PEI 25 Kda (μg) 30 187.5 15 30
Scale Spin tube®50 bioreactor 25 mL-Shake flask Spin tube ®50 bioreactor
  1. *w/o= without and w= with
  2. Transfection efficiency was monitored at 48 hpt by fluorescence microscope and flow cytometer. Cell count and viability were determined by Cedex analyzer
Back to article page

BMC Proceedings

ISSN: 1753-6561

Contact us