22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Cell Based Technologies: Abstracts of the 22^nd ESACT Meeting 2011 in Vienna

Authors: Hansjörg Hauser

Highly efficient, chemically defined and fully scalable biphasic production of vaccine viruses

Authors: Ingo Jordan and Volker Sandig

Recombinant antibody mixtures; optimization of cell line generation and single-batch manufacturing processes

Authors: Søren K Rasmussen, Lars S Nielsen, Christian Müller, Thomas Bouquin, Henrik Næsted, Nina T Mønster, Frank Nygaard, Dietmar Weilguny, Torben P Frandsen and Anne B Tolstrup

Cell-based medicinal products and the development of GMP-compliant processes and manufacturing

Authors: Luca Romagnoli, Ilaria Giuntini, Marta Galgano, Chiara Crosta, Luigi Cavenaghi and Maria Luisa Nolli

Biosynthetic pathway deflection – a new cell line engineering approach

Authors: Hans Henning von Horsten, Thomas Rose and Volker Sandig

Fluorescence-based tools to improve biopharmaceutical process development

Authors: Tiago M Duarte, Manuel JT Carrondo, Paula M Alves and Ana P Teixeira

Towards rational engineering of cells: Recombinant gene expression in defined chromosomal loci

Authors: Kristina Nehlsen, Leonor da Gama-Norton, Roland Schucht, Hansjörg Hauser and Dagmar Wirth

Human hair follicle equivalents in vitro for transplantation and chip-based substance testing

Authors: R Horland, G Lindner, I Wagner, B Atac, S Hoffmann, M Gruchow, F Sonntag, U Klotzbach, R Lauster and U Marx

Chromosome identification and its application in Chinese hamster ovary cells

Authors: Yihua Cao, Shuichi Kimura, Joon-young Park, Miyuki Yamatani, Kohsuke Honda, Hisao Ohtake and Takeshi Omasa

Development of an automated, multiwell plate based screening system for suspension cell culture

Authors: Sven Markert and Klaus Joeris

Utilization of multifrequency permittivity measurements in addition to biomass monitoring

Authors: Christoph Heinrich, Tim Beckmann, Heino Büntemeyer and Thomas Noll

ATF4 over-expression increased IgG₁productivity in Chinese hamster ovary cells

Authors: Ahmad M Haredy, Akitoshi Nishizawa, Kohsuke Honda, Tomoshi Ohya, Hisao Ohtake and Takeshi Omasa

Characterization of novel pneumatic mixing for single-use bioreactor application

Authors: Brian Lee, David Fang, Matthew Croughan, Manuel Carrondo and Sang-Hoon Paik

Enhanced protein synthesis and secretion using a rational signal-peptide library approach as a tailored tool

Authors: Beate Stern, Asta Optun, Melanie Liesenfeld, Christine Gey, Markus Gräfe and Ian F Pryme

High-capacity assay to quantify the clonal heterogeneity in potency of mesenchymal stem cells

Authors: Kim C O’Connor, Katie C Russell, Donald G Phinney, Michelle R Lacey, Bonnie L Barrilleaux and Kristin E Meyertholen

Improvement of cell freezing technologies: towards a fully closed process

Authors: Aurore Polès-Lahille, Brigitte Lafuente, Virginie Perrier, David Balbuena and Didier Peyret

Disposable bioreactors: from process development to production

Authors: Aurore Polès-Lahille, Celine Richard, Sandrine Fisch, David Pedelaborde, Sandy Gerby, Nora Kadi, Virginie Perrier, Robert Trieau, David Balbuena, Laure Valognes and Didier Peyret

Neuroprotective activity of a new erythropoietin formulation with increased penetration in the central nervous system

Authors: Marina Etcheverrigaray, Natalia Ceaglio, Mónica Mattio, Marcos Oggero, Ignacio Amadeo, Guillermina Forno, Norma Perotti and Ricardo Kratje

New reporter cell clones to determine the biological activity of human type I interferons

Authors: Milagros Bürgi, Claudio Prieto, Marcos Oggero, Mariela Bollati-Fogolín, Marina Etcheverrigaray and Ricardo Kratje

Analytical techniques for characterization of raw materials in cell culture media

Raw materials are a critical part of any cell culture medium; therefore, it is of utmost importance to understand and characterize them for high-quality product. The raw material characterization (RMC) program...

Authors: Chandana Sharma, Barry Drew, Kevin Head, Rani Pusuluri and Matthew V Caple

Next-generation sequencing of the CHO cell transcriptome

Authors: Jennifer Becker, Christina Timmermann, Tobias Jakobi, Oliver Rupp, Rafael Szczepanowski, Matthias Hackl, Alexander Goesmann, Andreas Tauch, Nicole Borth, Johannes Grillari, Alfred Pühler, Thomas Noll and Karina Brinkrolf

A strategy to obtain recombinant cell lines with high expression levels. Lentiviral vector-mediated transgenesis

Authors: Claudio Prieto, Diego Fontana, Marina Etcheverrigaray and Ricardo Kratje

In-situ cell density monitoring and apoptosis detection in adherent Vero cell bioreactor cultures

Authors: Emma Petiot, Amal El-Wajgali, Geoffrey Esteban, Cécile Gény, Hervé Pinton and Annie Marc

Does earlier use of productivity enhancers during cell line selection lead to the identification of more productive cell lines?

Authors: Alison J Porter, Atul Mohindra, Juana Maria Porter and Andrew J Racher

Quantification of intracellular nucleotide sugars and formulation of a mathematical model for prediction of their metabolism

Authors: Ioscani Jiménez del Val, Judit M Nagy and Cleo Kontoravdi

Design and simulation of a controller system for metabolic shift regulation in mammalian cells

Authors: Damián Baeza, Ziomara P Gerdtzen and Cristian J Salgado

The way to a design space for an animal cell culture process according to Quality by Design (QbD)

Authors: Robert Puskeiler, Jan Kreuzmann, Caroline Schuster, Katharina Didzus, Nicole Bartsch, Christian Hakemeyer, Heike Schmidt, Melanie Jacobs and Stefan Wolf

Improvement of stem cell performance by supplementation with metabolic enhancers

Authors: Abi M Abitorabi and Christopher Wilcox

Use of Micro Bioreactor systems to streamline cell line evaluation and upstream process development for monoclonal antibody production

Authors: Steve R C Warr, Jai Patel, Rongzan Ho and Katy V Newell

Cell line selection using the Duetz Microflask system

Authors: Steve R C Warr, Sharon L White, Yuen-Ting Chim, Jai Patel and Hella Bosteels

Evaluation of an online biomass probe to monitor cell growth and cell death

Authors: Angelo Perani, Benjamin Gloria, Dongmao Wang, Anne-Sophie Buffier, Olivier Berteau, Geoffrey Esteban, Fiona E Smyth and Andrew M Scott

Impact on product quality of high productive GS-CHO cell lines

Authors: Angelo Perani, Benjamin Gloria, Dongmao Wang, Roger Murphy, Harjit Khangura Singh, Fiona E Smyth and Andrew M Scott

Anti-diabetes effect of water containing hydrogen molecule and Pt nanoparticles

Authors: Sanetaka Shirahata, Takeki Hamasaki, Keisuke Haramaki, Takuro Nakamura, Masumi Abe, Hanxu Yan, Tomoya Kinjo, Noboru Nakamichi, Shigeru Kabayama and Kiichiro Teruya

Metabolic enhancers: a new paradigm in cell culture media optimization

Authors: Abi M Abitorabi and Christopher Wilcox

Neuroprotective effects of 3,5-di-o-caffeoylquinic acid in vitro and in vivo

Authors: Junkyu Han and Hiroko Isoda

Improvement of insulin resistance by Cyanidin 3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression

Authors: Tetsuya Inaguma, Junkyu Han and Hiroko Isoda

Engineering CHO cell growth by stable manipulation of miRNA expression

Authors: Noëlia Sanchez, Nga Lao, Clair Gallagher, Martin Clynes and Niall Barron

Expression of recombinant human coagulation factors VII (rFVII) and IX (rFIX) in various cell types, glycosylation analysis, and pharmacokinetic comparison

Authors: Ernst Böhm, Michael Dockal, Michael Graninger, Meinhard Hasslacher, Martin Kaliwoda, Christian Konetschny, Artur Mitterer, Eva-Maria Muchitsch, Manfred Reiter and Friedrich Scheiflinger

Complex medium supplements optimized for the reduction of animal-derived components in vaccine production media

Authors: James F Babcock, Karen A Benedict and Amanda L Perlman

Application of complex and chemically-defined medium supplements toward cell line specific performance enhancement of biopharmaceutical production systems

Authors: James F Babcock, Karen A Benedict and Amanda L Perlman

A comparison of performance enhancing synergy among ultrafiltered yeast extracts and recombinant human serum albumin in CHO-K1 cells

Authors: James F Babcock, Karen A Benedict and Amanda L Perlman

Targeted metabolomics for bioprocessing

Authors: Denise Sonntag, Francesca M Scandurra, Torben Friedrich, Michael Urban and Klaus M Weinberger

Toolbox approach for fast generation of stable CHO production cell lines from different hosts

Authors: Susanne Seitz, Henning von Horsten, Thomas Rose, Volker Sandig and Karsten Winkler

Growth characterization of CHO DP-12 cell lines with different high passage histories

Authors: Christoph Heinrich, Timo Wolf, Christina Kropp, Stefan Northoff and Thomas Noll

Utilization of multifrequency permittivity measurements in addition to biomass monitoring

Authors: Christoph Heinrich, Tim Beckmann, Heino Büntemeyer and Thomas Noll

CHO cell lines generated by PiggyBac transposition

Authors: Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus and Florian M Wurm

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Authors: Sowmya Balasubramanian, Mattia Matasci, Lucia Baldi, David L Hacker and Florian M Wurm

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Authors: Divor Kiseljak, Yashas Rajendra, Sagar S Manoli, Lucia Baldi, David L Hacker and Florian M Wurm

Rapid recombinant protein production from pools of transposon-generated CHO cells

Authors: Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus and Florian M Wurm

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Authors: Yashas Rajendra, Divor Kiseljak, Lucia Baldi, David L Hacker and Florian M Wurm